A new method of identifying the peripheral blood and the menstrual blood.
- Author:
Ying-xi WANG
1
;
Xiao-jun ZHU
;
Zhang-ping JIAO
;
Zhi-yong LU
;
Wei-ni WANG
;
Ya-cheng LIU
;
Hui TANG
Author Information
1. School of Forensic Medicine, Shanxi Medical University, Taiyuan 030001, China. wangyingxi4722@163.com
- Publication Type:Journal Article
- MeSH:
Biomarkers;
Blood/metabolism*;
Female;
Gene Expression;
Gene Expression Profiling;
Humans;
Matrix Metalloproteinase 7/genetics*;
Menstruation/genetics*;
RNA, Messenger/genetics*;
Reverse Transcriptase Polymerase Chain Reaction;
beta-Defensins
- From:
Journal of Forensic Medicine
2012;28(5):359-361
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the tissue-specific gene expressions of the peripheral blood and the menstrual blood, and to search some specific factors to establish an effective method for identifying the peripheral blood and the menstrual blood.
METHODS:The specific products of the peripheral blood and the menstrual blood were detected by RT-PCR and separated by electrophoretic technology.
RESULTS:Beta-spectrin (SPTB) as one specific marker of peripheral blood and 18S rRNA as a kind of the housekeeping gene were expressed in both the peripheral blood and the menstrual blood. However, matrix metalloproteinase 7 (MMP7) as one specific marker of menstrual blood and human beta defensin 1 (HBD1) as one specific marker of vaginal discharge were only found in the menstrual blood.
CONCLUSION:There are differences of specific gene expressions between the peripheral blood and the menstrual blood. They could be accurately distinguished from each other by using the combination of fluorescence technology and RT-PCR to detect the specific identification of mRNA.
