LC-MS/MS analysis of determination of strychnine and brucine in formaldehyde fixed tissue.
- Author:
Lan-fen ZHAN
1
;
Ming-dong LIU
;
You-yi YAN
;
Yi YE
;
Wei WANG
;
Zhi-hui WANG
;
Jun-hong ZHAO
;
Lin-chuan LIAO
Author Information
1. West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China. lfz415@sohu.com
- Publication Type:Journal Article
- MeSH:
Chromatography, Liquid/methods*;
Forensic Toxicology;
Formaldehyde/chemistry*;
Formates;
Kidney/metabolism*;
Limit of Detection;
Liver/metabolism*;
Mass Spectrometry;
Molecular Structure;
Reproducibility of Results;
Spectrometry, Mass, Electrospray Ionization/methods*;
Strychnine/chemistry*;
Tandem Mass Spectrometry;
Tissue Distribution
- From:
Journal of Forensic Medicine
2012;28(5):347-350
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for determination of strychnine and brucine in formaldehyde fixed tissue by LC-MS/MS analysis.
METHODS:The samples were pretreated with solid phase extraction using SCX cartridges and separated on SB-C18 column with mobile phase 0.1% formic acid : 0.1% formic acid-acetonitrile (75:25). Electrospray ionization (ESI) source was utilized and operated in positive ion mode. Multiple reactions monitoring (MRM) mode was applied. External standard method was applied for quantitation.
RESULTS:The chromatographic separation of strychnine and brucine in formaldehyde fixed nephritic and hepatic tissues resulted successfully. The standard curve was linear in the range of 0.002-2.0 microg/g for strychnine and brucine in formaldehyde fixed tissues, and the correlation coefficient was more than 0.996. The limits of detection (LOD) of strychnine and brucine in nephritic tissues were 0.06ng/g and 0.03 ng/g, respectively. The LOD of both chemicals were 0.3 ng/g in hepatic tissues. The extraction recovery rate was more than 74.5%. The precision of intra-day and inter-day were both less than 8.2%.
CONCLUSION:Strychnine and brucine can be sensitive to be determined in formaldehyde fixed tissue by LC-MS/MS analysis. It can be applied in the forensic toxicological analysis.
