Changes of CK-MB and HSP 60 in electrical-injuried rats.
- Author:
Hui-tong LIU
1
;
Gao-wen FU
;
Ze ZHAO
;
Su-zhen DING
;
Qiao-feng WANG
;
Lei CHEN
;
Ya-nan XIE
;
Zhen-yuan WANG
Author Information
1. Medical College of Xi'an Jiaotong University, Xi'an 710061, China. liuhuitong@126.com
- Publication Type:Journal Article
- MeSH:
Animals;
Chaperonin 60/metabolism*;
Creatine Kinase, MB Form/metabolism*;
Electric Injuries/complications*;
Immunohistochemistry;
Liver/pathology*;
Myocardium/pathology*;
Rats
- From:
Journal of Forensic Medicine
2012;28(5):333-336
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the changes of creatine kinase-MB (CK-MB) and heat shock protein 60 (HSP 60) in rats without electric marks after electric injury, to identify the relationship of the CK-MB, HSP 60 and the time of electric injuries, and to evaluate the damage to cells after electric injury.
METHODS:The animal model of electric injury without electric marks was established by alternating current (voltage 110 V). Automatic biochemistry analyzer was used to detect the serum CK-MB and immunohistochemical staining technology was used to analyze the tissues of myocardium and left lobe of liver.
RESULTS:The amount of serum CK-MB was increased when the rats were injuried, and reached the peak at 30min. Then the amount of CK-MB began to decrease and showed a slight downward trend in 3-5 h after electric injury, and leveled off at 6 h. Immunohistochemistry staining also showed the changes of HSP 60 of rats' myocardial cells and hepatic cells regularly after electric injury.
CONCLUSION:The regular changes of serum CK-MB and tissular HSP 60 in rats can be used to diagnosis electric injury and assess the injury of internal organs after the electric injury without electric marks.
