Different expression of TNF-alpha in brain and peripheral organs after cerebral contusion of rats.
- Author:
Zhi YAN
1
;
Xiao-Li SUN
;
Yu-Lian HU
;
Min LIU
Author Information
1. West China School of Basic Medicine and Forensic Medicine, Sichuan University, Chengdu 610041, China. yanzhiyx2000@163.com
- Publication Type:Journal Article
- MeSH:
Animals;
Brain/metabolism*;
Brain Injuries/pathology*;
Contusions/pathology*;
Disease Models, Animal;
Female;
Immunohistochemistry;
Kidney/metabolism*;
Liver/metabolism*;
Lung/metabolism*;
Male;
Rats;
Rats, Sprague-Dawley;
Spleen/metabolism*;
Tissue Distribution;
Tumor Necrosis Factor-alpha/metabolism*
- From:
Journal of Forensic Medicine
2012;28(4):261-264
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To compare the expression of tumor necrosis factor-alpha (TNF-alpha) between brain and peripheral organs after cerebral contusion in order to provide the scientific theoretical basis for forensic pathological diagnosis and wound age estimation.
METHODS:Brain and peripheral organs including heart, liver, spleen, lung, kidney tissues of 45 SD rats after the cerebral contusion were obtained and TNF-alpha of these tissues were analyzed with immunohistochemistry methods.
RESULTS:TNF-alpha was detected at 1 h in brain, reaching maximum at 6 h and 3 d after the cerebral contusion, and then decreased but still kept at high expression level at 7 d. TNF-alpha was detected at 1 h after the cerebral contusion in heart, liver, spleen, lung and kidney tissues. The number of cells expressing TNF-alpha increased gradually, reaching maximum at 3 d after the contusion of brain, and then decreased but still kept at high expression level at 7 d.
CONCLUSION:Besides the change of cerebral contusion, this study considered both the brain and peripheral organs. It is helpful for forensic pathological diagnosis and wound age estimation after contusion of brain.
