Magnetic resonance diffusion tensor imaging for diagnosis of pyramidal tract damage in rats.
- Author:
Shang-Xun LI
1
;
Dai SHAN
;
Yi-Jie DUAN
;
Jing-Jun XING
;
Yang DING
;
Yi-Wu ZHOU
Author Information
1. Department of Forensic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. lishangxun@gmail.com
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Amyloid beta-Protein Precursor/metabolism*;
Animals;
Anisotropy;
Axons/pathology*;
Brain/pathology*;
Brain Injuries/pathology*;
Diffusion Magnetic Resonance Imaging/methods*;
Disease Models, Animal;
Image Processing, Computer-Assisted;
Male;
Neurofilament Proteins/metabolism*;
Pyramidal Tracts/pathology*;
Rats;
Rats, Sprague-Dawley;
Severity of Illness Index;
Time Factors
- From:
Journal of Forensic Medicine
2012;28(4):256-268
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the applicability of magnetic resonance diffusion tensor imaging (DTI) for diagnosis of pyramidal tract damage in rats.
METHODS:Marmarou's model was set up, followed by DTI scanning at 3, 12, 24 and 72 h post trauma to acquire the dispersion parameter of bilateral pyramidal tracts. Moreover, axonal varicosities per square millimeter and the percentage of positive area of axons demonstrated by beta-amyloid precursor protein (beta-APP) immunostaining were obtained, as well as the mean density and sum density of neurofilament (NF) 68 immunostaining.
RESULTS:Axial diffusivity (AD), fraction anisotropy (FA) and relative anisotropy (RA) in the pyramidal tract were significantly and continuously reduced and reached to the bottom at 72h post trauma (P < 0.05) in accord with the gradient of axonal damage verified by beta-APP and NF68 immunostaining. Furthermore, the changes of AD, FA and RA showed a significant negative correlation with the beta-APP immunohistochemical results.
CONCLUSION:DTI has important value for early diagnosis in pyramidal tract damage.
