Identification of sibling brothers using STR and Y-biallelic markers.
- Author:
Mei-yun TANG
1
;
Jian HUANG
;
Jin-hong CAI
;
Xiao-song HUANG
;
Wei XU
;
Zhi-xiong QU
;
Fei-bao SHAN
;
Hui-ling LU
Author Information
1. Furong Forensic Science Identification Center of Hunan Second People's Hospital, Changsha 410007, China. tangmeiyun2000@yahoo.com.cn
- Publication Type:Journal Article
- MeSH:
Alleles;
Chromosomes, Human, Y/genetics*;
Forensic Genetics/methods*;
Gene Frequency;
Genetic Loci/genetics*;
Genetic Markers;
Genotype;
Humans;
Male;
Microsatellite Repeats/genetics*;
Mutation;
Polymerase Chain Reaction/methods*;
Polymorphism, Genetic;
Siblings
- From:
Journal of Forensic Medicine
2012;28(3):190-194
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the methods for identification of sibling brothers with Y-STR locus mutation by detection of genetic markers on autosome and Y-biallelic.
METHODS:Goldeneye 20A and 18NC kit were used to genotyped the 35 STRs on autosome from two men. PowerPlex Y kit and Yfiler kit were used to genotyped the 16 STRs on Y chromosome full sibling index was calculated by ITO method. Twenty Y-biallelic markers were genotyped by fragment length discrepant allele specific PCR or general PCR.
RESULTS:Relationship of sibling brothers was found to have mutation of 2 loci on 16 Y-STR and the identical genetype of 20 Y-biallelic markers as well as a cumulative full sibling index of 4.3149 x 10(6) from 35 STRs on autosome.
CONCLUSION:In identification of paternal linage of Y-STR mutation, more genetic information can be acquired by detection of Y-biallelic markers including SNP and InDel.
