Effects of ketamine and alcohol on learning and memory impairment in mice.
- Author:
Mei-Yu YANG
1
;
Fei DING
;
Xiao-Gang JIANG
;
Xie-Xing WU
;
Zhen-Lun GU
;
Ci-Yi GUO
;
Shi-Zhong BIAN
Author Information
1. Institute of Forensic Science of Soochow University, Department of Forensic Medicine, Medical College of Soochow University, Suzhou 215123, China. meiyu0307@163.com
- Publication Type:Journal Article
- MeSH:
Acetylcholine/metabolism*;
Alcohols/pharmacology*;
Animals;
Brain/physiopathology*;
Drug Synergism;
Ketamine/pharmacology*;
Male;
Maze Learning/drug effects*;
Memory/drug effects*;
Memory Disorders/physiopathology*;
Mice;
Mice, Inbred ICR;
Serotonin/metabolism*;
Spatial Behavior/drug effects*
- From:
Journal of Forensic Medicine
2012;28(2):115-119
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the effects of ketamine and alcohol on learning and memory in mice and its possible mechanism.
METHODS:Forty mice were divided into 4 groups: normal control group, ketamine group, alcohol group, and alcohol plus ketamine group. Ketamine and alcohol were given by intraperitoneal injection and intragastric administration, respectively, 1 time per day, for 14 days. The ability of learning and memory in mice was tested by the method of step-down and Morris water maze. Acetylcholine (ACh) and 5-hydroxy tryptamine(5-HT) in mice brain tissue were analyzed for the possible mechanism.
RESULTS:(1) Step-down: The treatment groups lessened the latency and added wrong times (P < 0.05). The number of errors in the combined treatment group significantly increased comparing with the single drug treatment group (P < 0.05). (2) Morris water-maze: The treatment groups prolonged the latency (P < 0.05), reduced the target quadrant activity time significantly (P < 0.05), and decreased the numbers of crossing the former platform significantly (P < 0.05). (3) Biochemical index determination: The concentrations of ACh and 5-HT in treatment groups decreased significantly (P < 0.05), showed a more decreasement comparing with the single drug treatment group.
CONCLUSION:Ketamine has a synergistic effect with alcohol on learning and memory impairment in mice, which may be related to the common inhibitive effect on the ACh and 5-HT.
