Detection of matrix metalloproteinase-11 in menstrual blood by enhanced chemiluminescence method.
- Author:
Ya-Qing ZHANG
1
;
Hui-Ling LU
;
Ya-Nan YAO
Author Information
1. Forensic Judical Appraisal Institute, Blood Center of Hainan Province, Haikou 570311, China. fywzzyq2005@yahoo.com.cn
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Biomarkers/blood*;
Blood Stains;
Blotting, Western;
Female;
Forensic Medicine/methods*;
Humans;
Luminescent Measurements/methods*;
Matrix Metalloproteinase 11/blood*;
Menstruation;
Reproducibility of Results;
Saliva/chemistry*;
Sensitivity and Specificity;
Urine/chemistry*;
Vagina/chemistry*
- From:
Journal of Forensic Medicine
2012;28(2):109-111
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the forensic application value of detection of matrix metalloproteinase-11 (MMP-11) in menstrual blood by enhanced chemiluminescence method.
METHODS:Menstrual blood, vaginal swab, peripheral blood, saliva stain, urine stain and semen stain were collected to detect whether or not there were MMP-11 using enhanced chemiluminescence method. The specificity and reliability of the MMP-11 assay along with its sensitivity were evaluated.
RESULTS:The positive detection rate of MMP-11 in menstrual blood was 89.47%, whereas no MMP-11 was found in vaginal swab, peripheral blood, saliva stain, urine stain and semen stain. When 25 microL sample was added, the mass concentration of protein was 1.329 microg/microL, then MMP-11 could be detected. A positive detection rate of 89.58% was observed in MMP-11 positive menstrual blood samples after stored at 4 degrees C for 20 months.
CONCLUSION:Enhanced chemiluminescence method is sensitive and specific for detecting MMP-11, and can be applied to distinguish menstrual blood from common stain such as peripheral blood, vaginal fluid.
