Establishment of skin scald model in mice.
- Author:
Peng REN
1
;
Da-Wei GUAN
;
Rui ZHAO
;
Wen-Xiangu MA
;
Shu-Tao ZHANG
Author Information
1. Department of Forensic Pathology, School of Forensic Medicine, China Medical University, Shenyang 110001, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Burns/pathology*;
Disease Models, Animal;
Epithelium/pathology*;
Female;
Hot Temperature/adverse effects*;
Male;
Mice;
Random Allocation;
Reproducibility of Results;
Severity of Illness Index;
Skin/pathology*;
Time Factors
- From:
Journal of Forensic Medicine
2012;28(2):92-99
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish the model of skin scald in mice for the study of skin thermal injuries.
METHODS:After anaesthetization mice were scalded in a 1 cm-diameter circle area on the central dorsum by boiling water at contact times: 10s or 25s. The mice were sacrificed at 1, 3, 5, 7 and 10 days after scald. The skin samples were collected and analyzed by gross and histopathological examinations.
RESULTS:Deep II degree thermal injury involving full-thickness skin was observed in the 10s scald group. III degree thermal injury involving full-thickness skin and the dorsal skeletal muscle was observed in the 25 s scald group.
CONCLUSION:A mouse skin scald model is established which is stable and can be used on the skin thermal injury in future research.
