Research status and prospects of DNA test on difficult specimens.
- Author:
Hua-Wei DANG
1
;
Jiong MAO
;
Hui WANG
;
Jiang-Ping HUANG
;
Xiao-Gang BAI
Author Information
1. Institute of Forensic Science, Chengdu Public Security Bureau, Chengdu 610081, China. danghuawei@yahoo.cn
- Publication Type:Review
- MeSH:
Biomarkers;
Body Fluids/chemistry*;
DNA/genetics*;
DNA Fingerprinting/methods*;
DNA, Mitochondrial/genetics*;
Forensic Medicine/methods*;
Genome, Human;
Humans;
Laser Capture Microdissection/methods*;
Microsatellite Repeats;
Nucleic Acid Amplification Techniques/methods*;
Polymerase Chain Reaction/methods*;
Reproducibility of Results;
Sample Size
- From:
Journal of Forensic Medicine
2012;28(1):52-54
- CountryChina
- Language:Chinese
-
Abstract:
This paper reviews the advances of DNA detection on three types of difficult biological specimens including degraded samples, trace evidences and mixed samples. The source of different samples, processing methods and announcements were analyzed. New methods such as mitochondrial test system, changing the original experimental conditions, low-volume PCR amplification and new technologies such as whole genome amplification techniques, laser capture micro-dissection, and mini-STR technology in recent years are introduced.
