Screening and confirmation of psychotropic drugs in blood and urine by HPLC-LTQ Orbitrap MS.
- Author:
Xiao-Wen LI
1
;
Bao-Hua SHEN
;
Zheng JIANG
;
Xian-Yi ZHUO
Author Information
1. Department of Forensic Medicine, Shanghai Medical College, Fudan University, Shanghai 200032, China. 09211010055@fudan.edu.cn
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Chromatography, High Pressure Liquid/methods*;
Forensic Toxicology;
Humans;
Mass Spectrometry/methods*;
Molecular Structure;
Molecular Weight;
Psychotropic Drugs/urine*;
Reproducibility of Results;
Sensitivity and Specificity;
Substance Abuse Detection/methods*
- From:
Journal of Forensic Medicine
2012;28(1):44-48
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a screening and confirmation method for psychotropic drugs and their metabolites in human blood and urine by HPLC-LTQ Orbitrap MS.
METHODS:The samples were pretreated with Sirocco protein precipitation plate, and then analyzed by HPLC-LTQ Orbitrap MS. The method was validated in terms of the limit of detection (LOD). An accurate mass database was created for psychotropic drugs screening.
RESULTS:The LOD for most of 56 determined compounds was < or = 0.1 ng/mL. The accurate mass database included the accurate mass information of 61 psychotropic drugs.
CONCLUSION:The method is accurate, rapid, sensitive and the database is suitable for psychotropic drugs screening and confirmation.
