Degenerate oligonucleotide primed-PCR technology establishment and its sensitivity test analysis.
- Author:
Jian-Qiang DENG
1
;
Bao-Qin LIU
;
Ji-Feng CAI
;
Wen-Hui LI
;
Ren LONG
;
Yi-Ping HOU
Author Information
1. Department of Forensic Medicine, Hainan Medical College, Haikou 570102, China. forensicpatho@163.com
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
DNA/genetics*;
DNA Fingerprinting/methods*;
DNA Primers;
Electrophoresis, Capillary;
Gene Amplification;
Genotype;
Humans;
Nucleic Acid Amplification Techniques;
Oligonucleotides/genetics*;
Polymerase Chain Reaction/methods*;
Reproducibility of Results;
Sensitivity and Specificity;
Tandem Repeat Sequences
- From:
Journal of Forensic Medicine
2012;28(1):41-43
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a whole genome amplification testing system based on degenerate oligonucleotide primed-PCR (DOP-PCR) and to explore its reliability and sensitivity.
METHODS:DOP-PCR amplified production was detected by fluorescent labeled multiplex STR amplification and capillary electrophoresis detection system to determine reliability and sensitivity of DOP-PCR system.
RESULTS:DOP-PCR system was successfully established and the detection sensitivity reached 5 cells (30 pg) by pretreatment of DOP-PCR and then detection of STR genotyping.
CONCLUSION:The system established in this study is reliable and more testing sensitive for forensic trace evidence.
