The number of circulating fibrocytes of skeletal muscle in rats after contusion.
- Author:
Li-Qiang LI
1
;
Tian-Shui YU
;
Da-Wei GUAN
;
Yan-Yan FAN
;
Rui ZHAO
;
Wen-Xiang MA
;
Shu-Tao ZHANG
;
Shu ZHANG
;
Peng REN
;
Jiao-Yong LI
Author Information
1. Department of Forensic Pathology, School of Forensic Medicine, China Medical University, Shenyang 110001, China.
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Animals;
Biomarkers/metabolism*;
Collagen Type I/metabolism*;
Contusions/pathology*;
Disease Models, Animal;
Forensic Pathology;
Immunohistochemistry;
Leukocyte Common Antigens/metabolism*;
Male;
Mesenchymal Stem Cells/metabolism*;
Microscopy, Fluorescence;
Muscle, Skeletal/pathology*;
Random Allocation;
Rats;
Rats, Sprague-Dawley;
Time Factors;
Wound Healing
- From:
Journal of Forensic Medicine
2012;28(1):1-11
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTION:To investigate the time-dependent appearance of circulating fibrocytes of skeletal muscle in rats after contusion.
METHODS:The model of skeletal muscle wound was established in rat. The circulating fibrocytes in contused skeletal muscle were detected by CD45 and procollagen I double immunofluorescence staining method.
RESULTS:In the control group, CD45- and procollagen I-positive cells were not detected in skeletal muscle. A few CD45 cells were observed aged from 6 h to 1 d after contusion. A few CD45- and procollagen I-positive cells (fibrocytes) initially gathered in injury area 3d after injury. The ratio of positive fibrocytes significantly increased 5 d after injury. The ratio of fibrocytes was highest at 7 d after contusion and then decreased. The volume of fibrocytes showed bigger with injury time increase compared with 3 d group. The expression of procollagen I and CD45 were weakened at 14d after injury.
CONCLUSION:The circulating fibrocytes are detected in contused skeletal muscle in time-dependent pattern. Circulating fibrocytes may be a marker in the wound age determination for contused skeletal muscle.
