Rapid DNA identification using 6+1 STR kit and EX-Q20 electrophoresis.
- Author:
Yuan PING
1
;
Huai-Gu ZHOU
;
Yan XU
;
Zi-Fang XIA
;
Wei-Guo ZHENG
Author Information
1. Shanghai Key Laboratory of Crime Scene Evidence, Institute of Forensic Science, Shanghai Public Security Bureau, Shanghai 200083, China. pingyuan803@gmail.com
- Publication Type:Journal Article
- MeSH:
Alleles;
Blood Stains;
DNA/genetics*;
DNA Fingerprinting/methods*;
DNA Primers;
Electrophoresis, Capillary/methods*;
Forensic Medicine/methods*;
Genotype;
Genotyping Techniques/methods*;
Humans;
Polymerase Chain Reaction/methods*;
Reagent Kits, Diagnostic;
Reproducibility of Results;
Tandem Repeat Sequences
- From:
Journal of Forensic Medicine
2011;27(6):444-446
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a rapid STR genotyping method for individual identification.
METHODS:Two hundred blood samples from FTA were collected. Equal amount of blood were collected by puncher and analyzed using two methods (6+1 STR kit in combination with EX-Q20 electrophoresis and Sinofiler kit in combination with POP4 electrophoresis). Consuming time and results of two methods were compared.
RESULTS:6+1 STR kit in combination with EX-Q20 electrophoresis method can obtain all genotyping results and be shorter time.
CONCLUSION:6+1 STR kit in combination with EX-Q20 electrophoresis method is used to STR genotyping with accurate, reliable results and this new method is potential value in mass personnel investigation and comparison in major criminal cases. It also can raise the work efficiency.
