Determination of propofol in human blood by GC-MS.
- Author:
Ying-Ying SUN
1
;
Ping XIANG
;
Wei LIU
;
Jun BU
;
Min SHEN
Author Information
1. Shanghai Key Laboratory of Forensic Medicine, Institute of Forensic Science, Ministry of Justice, PR China, Shanghai 200063, China. sunyingying1986@sina.com
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Anesthetics, Intravenous/poisoning*;
Drug Monitoring/methods*;
Forensic Toxicology/methods*;
Gas Chromatography-Mass Spectrometry/methods*;
Humans;
Male;
Molecular Structure;
Propofol/poisoning*;
Reproducibility of Results;
Sensitivity and Specificity
- From:
Journal of Forensic Medicine
2011;27(6):430-433
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a gas chromatography-mass spectrometry (GC-MS) method for determination of propofol in human blood.
METHODS:Propofol and 2-(tert-Butyl)-4,6-dimethylphenol (internal standard) were isolated from human blood samples with liquid-liquid ether extraction. The organic layer was collected after centrifugation and dried using the water bath. The extracted residue was analyzed by GC-MS.
RESULTS:Propofol and the internal standard showed a good separation with a good linear concentration ranged from 0.02 to 10 microg/mL in blood. The linear function was y = 0.313 6 x-0.006 8 with the correlation coefficient of 0.9997. The precision of intra-day and inter-day were less than 4.8% and the lower limit of detection of propofol was 0.005 microg/mL. Propofol concentration of blood was 0.14 microg/mL using this method in the practice work.
CONCLUSION:The GC-MS method is rapid, sensitive, reliable and suitable for qualitative and quantitative analysis propofol of blood in forensic toxicological analysis and clinical drug monitoring.
