Analysis of the changes of amide A in rabbit heart after death by FTIR spectroscopy.
- Author:
Hai-Dong ZHANG
1
;
Tian-Tong YANG
;
Na ZHENG
;
Ting-Yi SUN
;
Liang LIU
Author Information
1. Key Laboratory of Evidence Science, China University of Political Science and Law, Ministry of Education, Beijing 100040, China. zhd_zzw@126.com
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Amides/metabolism*;
Animals;
Female;
Forensic Pathology/methods*;
Male;
Myocardium/metabolism*;
Postmortem Changes;
Rabbits;
Regression Analysis;
Spectroscopy, Fourier Transform Infrared;
Time Factors
- From:
Journal of Forensic Medicine
2011;27(5):321-323
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the relationship of changes of amide A in rabbit heart and the postmortem interval (PMI) by FTIR spectroscopy technique.
METHODS:Thirty-two rabbits were sacrificed and the hearts were sampled at 20 degrees C within 48 h postmortem points. All samples were sliced and tested by FTIR spectroscopy technique. The images of amide A were created by FTIR spectroscopic imaging. The positive and negative area ratios of amide A were analyzed using imaging analysis system.
RESULTS:The positive and negative area ratios declined regularly with the prolongation of death time in 48 h. There was a significant quadric relationship between the area ratios (y) of amide A (positive and negative area) and PMI(x). The regression equation was y = 0.001x2-0.038x + 0.747(R2 = 0.940).
CONCLUSION:The ratios of positive and negative area of amide A showed a strong correlation with PMI and could be used to estimate PMI.
