Simultaneous determination of trihexyphenidyl, chlorpromazine and clozapine in blood by GC-MS.
- Author:
Shui-Qing ZHENG
1
;
Wei WANG
;
Chen LIANG
;
Rong WANG
;
Fei-Jun GONG
;
Zhong-Ping WU
;
Yong-Sheng CHEN
;
Yu-Rong ZHANG
;
Run-Sheng ZHANG
Author Information
1. Shanghai Key Laboratory of Crime Scene Evidence, Institute of Forensic Science, Shanghai Public Security Bureau, Shanghai 200083, China.
- Publication Type:Journal Article
- MeSH:
Adult;
Antipsychotic Agents/poisoning*;
Chlorpromazine/blood*;
Clozapine/blood*;
Female;
Forensic Toxicology;
Gas Chromatography-Mass Spectrometry/methods*;
Humans;
Hydrogen-Ion Concentration;
Male;
Middle Aged;
Reproducibility of Results;
Sensitivity and Specificity;
Solvents/chemistry*;
Trihexyphenidyl/blood*
- From:
Journal of Forensic Medicine
2011;27(4):271-273
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To develop a method to measure trihexyphenidyl, chlorpromazine and clozapine in human blood with GC-MS.
METHODS:The specimens were alkalized (pH > 10) and extracted with V (benzene):V(ethyl acetate) = 1:1, and qualitatively analyzed using GC-MS-Full Scan with internal standard SKF525A. The specimens were alkalized (pH > 10) and extracted with V(benzene):V(ethyl acetate) = 1:1, and quantitatively analyzed using GC-MS-SIM with internal standard diazepam-d5.
RESULTS:The lowest detection limits of trihexyphenidyl, chlorpromazine and clozapine were 0.3, 0.3 and 0.7 ng/mL (S/N > or = 3) respectively. The calibration curve in 20-10 000 ng/mL showed a good linear distribution. The recovery rate was 79.9% to 85.5%. The RSDs of intraday and interday were less than 5.1%.
CONCLUSION:The established method was simple, sensitive and accurate for simultaneous determination of trihexyphenidyl, chlorpromazine and clozapine in human blood, and can be applied in forensic toxicological cases.
