Expression of HSP70 mRNA in hippocampus of rats after diffuse axonal injury.
- Author:
Feng WANG
1
;
Yong-Hong LI
Author Information
1. Department of Forensic Medicine, Wannan Medical College, Wuhu 241001, China. wangmaster76@163.com
- Publication Type:Journal Article
- MeSH:
Animals;
Axons/pathology*;
Diffuse Axonal Injury/pathology*;
Disease Models, Animal;
Forensic Pathology;
HSP70 Heat-Shock Proteins/metabolism*;
Hippocampus/metabolism*;
Male;
RNA, Messenger/metabolism*;
Random Allocation;
Rats;
Rats, Wistar;
Reverse Transcriptase Polymerase Chain Reaction;
Time Factors
- From:
Journal of Forensic Medicine
2011;27(4):250-252
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the changes of expression of heat-shock proteins (HSP) 70 mRNA in hippocampus of rats after diffuse axonal injury (DAI).
METHODS:RT-PCR products of HSP70 mRNA were tested by agarose gel electrophoresis after RT-PCR amplification. The changes of HSP70 mRNA expression were observed in rat hippocampus after DAI.
RESULTS:The expression of HSP70 mRNA in the hippocampus could be detected 4 h after DAI. It reached maximum at 24 h and declined after 48 h.
CONCLUSION:HSP70 mRNA in hippocampus of rats could be useful for diagnosis of DAI.
