Time-dependent recruitment and differentiation of fibrocytes in mouse skin wound healing.
- Author:
Yan-Yan FAN
1
;
Da-Wei GUAN
;
Tao WANG
;
Wei-Wei LIU
;
Ji-Long ZHENG
;
Zhen-Bin ZHAO
;
Tian-Shui YU
;
Wen-Xiang MA
Author Information
1. School of Forensic Medicine, China Medical University, Shenyang 110001, China. yyfan998@yahoo.com
- Publication Type:Journal Article
- MeSH:
Animals;
Cell Count;
Cell Differentiation;
Disease Models, Animal;
Fibroblasts/metabolism*;
Leukocyte Common Antigens/metabolism*;
Male;
Mice;
Mice, Inbred BALB C;
Myofibroblasts/metabolism*;
Skin/pathology*;
Staining and Labeling;
Time Factors;
Wound Healing
- From:
Journal of Forensic Medicine
2011;27(4):246-249
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the time-dependent recruitment and differentiation of fibrocytes in skin wound healing.
METHODS:Fibrocytes (expressing CD45 and procollagen I ) and myofibroblasts (expressing CD45 and alpha-SMA) were co-localized by immunofluorescent staining. The number of fibrocytes and myofibroblasts was counted at different post-wounding interval.
RESULTS:At 3 d after injury, fibrocytes started to recruit at the margin of the wounds. At 5 d after injury, myofibroblasts started to appear in new formed granulation tissue. The number of fibrocytes and myofibroblasts peaked at 7 d post-wounding.
CONCLUSION:During skin wound healing, myofibroblasts in granulation tissue originated at least partly from fibrocytic differentiation. The time-dependent recruitment and differentiation of fibrocytes may provide new information for wound age determination.
