Stability of estazolam in dog tissues preserved in formaldehyde solution.
- Author:
Jiu-xi YUAN
1
;
Yu-jin WANG
;
Ke-ming YUN
Author Information
1. School of Forensic Medicine, Shanxi Medical University, Taiyuan 030001, China. yuanjiuxi@sina.com
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Administration, Oral;
Animals;
Brain Chemistry;
Chromatography, High Pressure Liquid;
Dogs;
Drug Stability;
Estazolam/poisoning*;
Forensic Toxicology/methods*;
Formaldehyde;
Hypnotics and Sedatives/poisoning*;
Kidney/chemistry*;
Liver/chemistry*;
Male;
Solutions;
Time Factors;
Tissue Preservation/methods*
- From:
Journal of Forensic Medicine
2011;27(2):117-124
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the stability of estazolam in biological samples preserved in formaldehyde solution.
METHODS:The dog was given intragastric administration of estazolam with a dose of 37.6 mg/kg and killed 2 h later. Heart, liver, kidney and brain of the dog were cut up into 1 g and preserved in 4% formaldehyde solution respectively. The content of estazolam in biological samples and formaldehyde solution were analyzed by HPLC at different times.
RESULTS:The content of estazolam in heart, liver, kidney and brain or in formaldehyde solution reduced gradually followed with the extention of preservation time. At the 63rd day, estazolam content in four tissues were 0.8%, 1.7%, 1.0% and 2.2% of the original content respectively.
CONCLUSION:Estazolam in tissues can diffuse into formaldehyde solution and decomposed quickly, so biological samples contained estazolam should not be preserved in formaldehyde solution.
