The expression of VCAM-1 in organs of rats died of anaphylactic shock.
- Author:
Xiao-jie LI
1
;
Xiang-jie GUO
;
Cai-rong GAO
Author Information
1. School of Forensic Medicine, Shanxi Medical University, Taiyuan 030001, China.
- Publication Type:Journal Article
- MeSH:
Anaphylaxis/pathology*;
Animals;
Antibodies, Monoclonal/therapeutic use*;
Brain/metabolism*;
Disease Models, Animal;
Female;
Immunohistochemistry;
Kidney/metabolism*;
Lung/metabolism*;
Male;
Random Allocation;
Rats;
Rats, Sprague-Dawley;
Time Factors;
Vascular Cell Adhesion Molecule-1/metabolism*
- From:
Journal of Forensic Medicine
2011;27(2):87-90
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect and expression of the vascular cell adhesion molecule-1 (VCAM-1) in organs of rats died of anaphylactic shock.
METHODS:The models of anaphylactic shock in rats were made and the immunohistochemistry of SABC was used to detect as follows: (1) The expression of VCAM-1 in rat lung, heart, brain, kidney, liver, spleen, stomach and intestine. (2) VCAM-1 levels in lungs at 10 min, 30 min after the allergic shock, and the time of death. (3) VCAM-1 levels in lungs of rats after the intervention of anti-VCAM-1.
RESULTS:After the death, the expression VCAM-1 in lungs increased significantly relative to the control group and followed the extension of shock. In the rats which were injected with the anti-VCAM-1, the expression of VCAM-1 in lungs reduced.
CONCLUSION:(1) The expression of VCAM-1 shows difference in the various organs of rats after anaphylactic shock. The change of VCAM-1 is the most obvious in lungs and would increase followed the extension of anaphylactic shock. (2) After the anaphylactic shock, anti-VCAM-1 can inhibit the expression of VCAM-1 in rat lung.
