Progress on DNA quantification in estimation of postmortem interval.
- Author:
Xu LIN
1
;
Ya-sha YIN
;
Qiang JI
Author Information
1. Department of Forensic Medicine, School of Basic Medical Science, Nanjing Medical University, Nanjing 210029, China.
- Publication Type:Review
- MeSH:
Animals;
Cell Nucleus/metabolism*;
Comet Assay/methods*;
DNA/analysis*;
DNA Degradation, Necrotic;
Flow Cytometry;
Forensic Pathology;
Humans;
Image Processing, Computer-Assisted;
Kidney/pathology*;
Postmortem Changes;
Rosaniline Dyes;
Spleen/pathology*;
Temperature;
Time Factors
- From:
Journal of Forensic Medicine
2011;27(1):47-53
- CountryChina
- Language:Chinese
-
Abstract:
Estimation of postmortem interval (PMI) is one of the difficult problems in forensic medicine. With the development of molecular biological techniques, DNA quantification methods were widely applied in estimating PMI. The postmortem degradation of DNA in different tissues and organs was discussed in this article and the recent DNA quantitative techniques being used for estimating PMI were reviewed. These techniques included single cell gel electrophoresis, Feulgen staining image analysis, flow cytometry.
