Concentration changes of potassium and hypoxanthine in vitreous humor of swine and its application to postmortem interval estimation.
- Author:
Chao FANG
1
;
Song-cai WANG
;
Li-min SUN
;
Xiao-ting ZHANG
;
Wei-qing LONG
;
Hua-lan JING
Author Information
1. Department of Forensic Pathology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China. fch76@126.com
- Publication Type:Journal Article
- MeSH:
Animals;
Chromatography, High Pressure Liquid/methods*;
Female;
Forensic Pathology;
Hypoxanthine/analysis*;
Male;
Postmortem Changes;
Potassium/analysis*;
Regression Analysis;
Sodium/analysis*;
Swine;
Temperature;
Time Factors;
Vitreous Body/chemistry*
- From:
Journal of Forensic Medicine
2011;27(1):9-21
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the relationship between postmortem interval (PMI) and concentration changes of components in swine vitreous humor.
METHODS:Ninety-six porcine eyes from swine dying from acute massive hemorrhage, being randomly divided into 24 groups, were stored in dark situation, at temperature of (15 +/- 2) degrees C and humidity of (50 +/- 5)% for 2-96 hours separately. The vitreous humor was collected. Concentrations of K+, Na+, Cl- and hypoxanthine (Hx) were analyzed by automatic biochemical analyzer and ultra performance liquid chromatograph (UPLC). The data were statistically analyzed by SPSS software.
RESULTS:Linear regression analysis showed that concentrations of vitreous K+ and Hx were positively correlated with PMI(R2=0.767 and R2 = 0.793, respectively). Binary linear regression showed a higher correlation for K+ and Hx with PMI estimation (R2 = 0.866). PMI was not significantly correlated with vitreous Na+ and Cl- concentrations.
CONCLUSION:Vitreous K+ and Hx concentrations can be used as the objective markers for PMI estimation. The binary linear regression functions of vitreous K+ and Hx concentrations with PMI are more accurate for estimating the PMI.
