Determination of TTX in biological samples by LC-MS/MS.
- Author:
Qing DA
1
;
Wei LIU
;
Bao-hua SHEN
;
Min SHEN
Author Information
1. Department of Forensic Medicine, Medical College of Soochow University, Suzhou 215123, China. darcy5358@sina.com
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Animals;
Body Fluids/chemistry*;
Chromatography, Liquid/methods*;
Female;
Forensic Toxicology;
Humans;
Liver/chemistry*;
Reproducibility of Results;
Sensitivity and Specificity;
Solid Phase Extraction;
Specimen Handling/methods*;
Swine;
Tandem Mass Spectrometry/methods*;
Tetrodotoxin/urine*
- From:
Journal of Forensic Medicine
2010;26(6):432-435
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To propose a method for determination of tetrodotoxin (TTX) in human blood, urine and liver by liquid chromatography with tandem mass spectrometry (LC-MS/MS).
METHODS:Solid phase extraction is used after the samples are precipitated, then the samples will be analyzed by LC-MS/MS.
RESULTS:The limits of detection were 2 ng/mL in blood and urine and 4 ng/g in liver for TIX respectively, the linear correlation coefficients were not less than 0.9973, both of the intra-day and inter-day precisions were less than 12.80%, the recoveries for all kinds of specimens were more than 47.2%.
CONCLUSION:This method is efficient, sensitive and accurate and was successfully validated for implementation in forensic toxicological analysis.
