The relationship between GAPDH mRNA degradation in the mouse liver and postmortem interval.
- Author:
Hong-yan WU
1
;
Ke-jie WANG
;
Lin ZHANG
;
Li-wei GUO
;
Juan-ning GUO
;
Ai-ying FAN
Author Information
1. Department of Forensic Medicine, School of Basic Medical Science, Xinxiang Medical University, Xinxi-ang 453003, China. whyfine@163.com
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Animals;
Female;
Forensic Pathology;
Glyceraldehyde-3-Phosphate Dehydrogenases/metabolism*;
Liver/metabolism*;
Male;
Mice;
Mice, Inbred Strains;
Postmortem Changes;
RNA Stability;
RNA, Messenger/metabolism*;
Regression Analysis;
Reverse Transcriptase Polymerase Chain Reaction;
Temperature;
Time Factors
- From:
Journal of Forensic Medicine
2010;26(6):425-427
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the relationship between degradation of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA in the mouse liver and postmortem interval (PMI).
METHODS:Sixty NIH mice were sacrificed by cervical dislocation and suffocation, and then placed into 10 degrees C and 25 degrees C temperature-controlling systems. The changes of GAPDH mRNA in the liver were detected by two-step fluorimetric reverse transcriptase polymerase chain reaction (RT-PCR) technique and nucleic acids protein cryoscope from 0 to 48 h postmortem.
RESULTS:In the mouse liver, the amplification products of GAPDH mRNA could be examined within 48 h postmortem in 10 degrees C temperature-controlling system and within 36 h postmortem in 25 degrees C temperature-controlling system. The amplification products showed a decreasing tendency.
CONCLUSION:Degradation of GAPDH mRNA in the mouse liver is negative correlation with PMI. GAPDH mRNA could be a new marker for estimation of PMI.
