Comparison of mtDNA extraction from different parts of sarcosaphagous insects.
- Author:
Ya-Dong GUO
1
;
Ji-Feng CAI
;
Ri-Na SU
;
Yun-Feng CHANG
;
Ling-Mei LAN
;
Xiang LI
;
Ji-Fang WEN
Author Information
1. Department of Forensic Medicine, School of Basic Medical Science, Central South University, Changsha 410013, China. gdy82@126.com
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Animals;
Coleoptera/genetics*;
DNA, Mitochondrial/isolation & purification*;
Diptera/genetics*;
Electron Transport Complex IV/genetics*;
Electrophoresis, Agar Gel;
Entomology;
Forensic Medicine/methods*;
Polymerase Chain Reaction/methods*;
Rabbits;
Sequence Analysis, DNA;
Species Specificity
- From:
Journal of Forensic Medicine
2010;26(5):336-339
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore mitochondrial DNA (mtDNA) extraction effects of different parts from sarcosaphagous insects using improved cetyltriethylammnonium bromide (CTAB) method.
METHODS:Thirteen Lucilia sericata (Meigen) and 13 Nicrophorus fossor (Erichson) were collected from the corpses of rabbits placed on the outdoor lawn in Huhehot district. Four parts (head, chest muscle, legs and wings) of insect were collected, and the mtDNA of all samples were extracted using CTAB method. The purity and concentration were tested using protein and nucleic acid spectrophotometry. The integrity of the extracted mtDNA and PCR products were checked by agarose gel electrophoresis. The PCR products were sequenced and the obtained sequences were imputed into GenBank for comparison.
RESULTS:mtDNA were successfully extracted from 10 head samples, 6 legs samples, 4 wing samples and 13 chest muscle samples of the Lucilia sericata (Meigen). Also, mtDNA were successfully extracted from 5 head samples, 8 legs samples, 3 wing samples and 13 chest muscle samples of the Nicrophorus fossor (Erichson).
CONCLUSION:mtDNA can be obtained from chest muscle and other parts of sarcosaphagous insects using the improved CTAB method.
