Immunohistochemical expression of HO-1 in traumatic human brain tissue.
- Author:
Xue PENG
1
;
Zhan-Feng JIN
;
Hong-Jiang SHAO
;
Tie-Lei GAO
;
Zhong LI
;
Ying-Hui ZHANG
Author Information
1. Department of Forensic Medicine, Harbin Medical University, Harbin 150081, China. flysnow990119@l26.com
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Adult;
Autopsy;
Brain/pathology*;
Brain Injuries/pathology*;
Case-Control Studies;
Female;
Forensic Pathology;
Heme Oxygenase-1/metabolism*;
Humans;
Immunohistochemistry;
Male;
Middle Aged;
Staining and Labeling;
Time Factors;
Young Adult
- From:
Journal of Forensic Medicine
2010;26(5):325-331
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the expression of heme oxygenase-1 (HO-1) at different intervals and to provide evidence for estimation on injury intervals after brain contusion in human.
METHODS:Twenty-four patients died of serious brain injury were assigned as injury group and 4 patients died of non-brain injury were served as control group. HO-1 expression was analyzed in brain tissue at different time intervals (3 h, 6-9 h, 12-24 h, 36 h-3d, 5-8d, 17-20d) by immunohistochemistry and auto-image analysis system.
RESULTS:The level of HO-1 expression started to increase in 3 h after brain contusion compared to the control group (P < 0.05). The level of HO-1 expression highest level in 12-24 h group, and maintained high level in 36 h-3 d, then decreased gradually.
CONCLUSION:The expression of HO-1 might be a strong evidence for human brain contusion time estimation.
