The suppression of c-fos in neuron apoptosis induced by heroin.
- Author:
Pei-Yan LU
1
;
Ze-Hong LI
;
Yong GUO
;
Hua-Lan JING
;
Wen-Ying LIN
;
Xiao-Shan LIU
Author Information
1. Department of Forensic Medicine, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China. lupeiyan_2001@tom.com
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Animals;
Animals, Newborn;
Apoptosis/drug effects*;
Blotting, Western;
Cells, Cultured;
Cerebellum/metabolism*;
Dose-Response Relationship, Drug;
Down-Regulation;
Gene Expression Regulation/drug effects*;
Heroin/pharmacology*;
Male;
Neurons/metabolism*;
Proto-Oncogene Proteins c-fos/metabolism*;
RNA, Messenger/metabolism*;
Rats;
Rats, Sprague-Dawley;
Reverse Transcriptase Polymerase Chain Reaction
- From:
Journal of Forensic Medicine
2010;26(5):321-324
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the changes of c-fos in apoptosis of cerebellar granular neuron of neonatal SD rats induced by heroin and the mechanisms of neuronal injury caused by heroin.
METHODS:Primary cerebellar granular neuron were cultured in vitro, the model of apoptosis induced by heroin was established. Reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting were adopted to investigate the changes of c-fos in cell models.
RESULTS:Ten microg/mL of heroin was the optimal dose to induce the apoptosis of cerebellar granular neuron at 48 h. Both Western blotting and RT-PCR showed down regulation of c-fos expression.
CONCLUSION:Heroin could induce apoptosis of cerebellar granular neuron and down regulation of c-fos, which may be one of the apoptosis mechanisms.
