Application of direct amplification with PowerPlex 16 HS system for detection of DNA in conventional evidence materials.
- Author:
Mi ZHOU
1
;
Tao TANG
;
Fan WEI
;
Han-qiu ZHANG
;
Xin-xin LI
;
Ren-jie DING
;
Jin ZHOU
;
Cheng-quan WANG
Author Information
1. Criminal Police Branch, Wuhu Public Security Bureau, Wuhu 241000, China. protease168@yahoo.com.cn
- Publication Type:Journal Article
- MeSH:
Blood Stains;
DNA/genetics*;
DNA Fingerprinting/methods*;
Forensic Genetics/methods*;
Humans;
Nucleic Acid Amplification Techniques/methods*;
Polymerase Chain Reaction/methods*;
Saliva/metabolism*;
Sensitivity and Specificity;
Tandem Repeat Sequences;
Tobacco
- From:
Journal of Forensic Medicine
2010;26(4):285-286
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the effectiveness of direct amplification with PowerPlex 16 HS system for DNA detection in three conventional materials: fresh blood, oral swab, and cigarette butt.
METHODS:The genetic data of 11 samples of fresh blood, 10 samples of oral swab and 10 samples of cigarette butts were analyzed with PowerPlex 16 HS kit. The result was statistically analyzed.
RESULTS:Success rate of whole genetic map test in fresh blood and oral swab samples was 100%. Success rate in cigarette butt samples was 90%.
CONCLUSION:Direct amplification with PowerPlex 16 HS system is effective method to three types of conventional evidence material including fresh blood, oral swab and cigarette butt.
