Construction and application of fluorescence labeled multiplex typing system for 3 new miniSTR loci.
- Author:
Bing DU
1
;
Ji-ping JIANG
;
Hong DU
;
Lin ZHANG
Author Information
1. Department of Forensic Science, North Sichuan Medical College, Nanchong 637000, China. dbing04@163.com
- Publication Type:Journal Article
- MeSH:
Alleles;
China/ethnology*;
DNA/metabolism*;
DNA Fingerprinting/methods*;
DNA Primers;
Forensic Genetics/methods*;
Gene Frequency;
Genotype;
Humans;
Nucleic Acid Amplification Techniques/methods*;
Polymorphism, Genetic;
Tandem Repeat Sequences
- From:
Journal of Forensic Medicine
2010;26(4):282-284
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a miniSTR multiplex set including three STR loci unlinked from the CODIS loci: D1S1676, D6S1274 and D17S1299, to generate amplified fragment less than 115 bp in size and to study the genotype of degraded DNA samples.
METHODS:After amplification with different fluorescence labeled primers, the amplified products from 100 unrelated individual and 2 highly degraded specimens were analyzed by 310 Genetic Analyzer.
RESULTS:Three miniSTR loci were determined by fluorescence-labeled multiplex-PCR technique. Each locus was successfully genotyped in all 100 samples. In D1S1676, D6S1274 and D17S1299 loci, 9, 9, 7 alleles and 27, 23, 18 genotypes were observed respectively. The distribution of genotype for three miniSTR loci in Chengdu Han population was in accordance with Hardy-Weinberg equilibrium. The combined exclusion probability and the combined discrimination power of the three STR loci in Chengdu Han population were 0.9991 and 0.9160 respectively.
CONCLUSION:This miniSTR multiplex set could be used in individual identification and paternity test. It also provides a new method in the analysis of degraded DNA sample.
