Application of multiple polymorphism genetic markers in determination of half sibling sharing a same mother.
- Author:
Ting-zhi QUE
1
;
Shu-min ZHAO
;
Cheng-tao LI
Author Information
1. Shanghai Key Laboratory of Forensic Medicine, Institute of Forensic Science, Ministry of Justice, PR China, Shanghai 200063, China. quetz@ssfjd.com.cn
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Chromosomes, Human, X/genetics*;
DNA, Mitochondrial/genetics*;
Female;
Forensic Genetics/methods*;
Genetic Markers;
Genotype;
Humans;
Male;
Polymorphism, Genetic;
Sequence Analysis, DNA;
Siblings;
Tandem Repeat Sequences/genetics*
- From:
Journal of Forensic Medicine
2010;26(4):279-300
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:Determination strategies for half sibling sharing a same mother were investigated through the detection of autosomal and X-chromosomal STR (X-STR) loci and polymorphisms on hypervariable (HV) region of mitochondrial DNA (mtDNA).
METHODS:Genomic DNA were extracted from blood stain samples of the 3 full siblings and one dubious half sibling sharing the same mother with them. Fifteen autosomal STR loci were genotyped by Sinofiler kit, and 19 X-STR loci were genotyped by Mentype Argus X-8 kit and 16 plex in-house system. Polymorphisms of mtDNA HV-I and HV-II were also detected with sequencing technology.
RESULTS:Full sibling relationship between the dubious half sibling and each of the 3 full siblings were excluded based on the results of autosomal STR genotyping and calculation of full sibling index (FSI) and half sibling index (HIS). Results of sequencing for mtDNA HV-I and HV-II showed that all of the 4 samples came from a same maternal line. X-STR genotyping results determined that the dubious half sibling shared a same mother with the 3 full siblings.
CONCLUSION:It is reliable to combine three different genotyping technologies including autosomal STR, X-STR and sequencing of mtDNA HV-I and HV-II for determination of half sibling sharing a same mother.
