Determination of ethyl glucuronide in blood and urine by LC-MS/MS.
- 作者:
Hui ZHAO
1
;
Xian-yi ZHUO
;
Hui YAN
;
Bao-hua SHEN
作者信息
1. Shanghai Medical College, Fudan University, Shanghai 200032, China.
- 出版类型:Research Support, Non-U.S. Gov't
- 医学主题词:
Alcoholism/urine*;
Biomarkers/urine*;
Chromatography, Liquid/methods*;
Ethanol/metabolism*;
Forensic Toxicology/methods*;
Glucuronates/urine*;
Humans;
Reproducibility of Results;
Sensitivity and Specificity;
Substance Abuse Detection/methods*;
Tandem Mass Spectrometry/methods*
- 来源:
Journal of Forensic Medicine
2010;26(4):269-272
- 国家China
- 语言:Chinese
-
摘要:
OBJECTIVE:To develop a method for determining ethyl glucuronide (EtG) in blood and urine by liquid chromatograph coupled with tandem mass spectrometer (LC-MS/MS).
METHODS:After blood and urine de-proteined by acetonitrile, the supernate obtained from a centrifuge was analyzed by LC-MS/MS.
RESULTS:Determination limit of EtG in both blood and urine was 0.05 pg/mL, with a linear range of 0.10-5.00 microg/mL (r > 0.999). Accuracy in both matrixes was 95%-109%. Inter- and intra-day RSD were less than 12%. The method showed an excellent performance when it was used to analyze authentic blood and urine samples for EtG.
CONCLUSION:The method is capable for blood and urine EtG analysis.
