Decomposition kinetics of bupivacaine in biological specimens.
- Author:
Yun LI
1
;
Tong-dan DU
;
Ke-ming YUN
Author Information
1. School of Forensic Medicine, Shanxi Medical University, Taiyuan 030001, China. liyun4378@yahoo.cn
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Anesthesia, Epidural;
Anesthetics, Local/urine*;
Animals;
Brain/metabolism*;
Bupivacaine/urine*;
Dogs;
Female;
Gas Chromatography-Mass Spectrometry/methods*;
Kinetics;
Male;
Temperature;
Time Factors;
Tissue Preservation/methods*
- From:
Journal of Forensic Medicine
2010;26(4):266-268
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study on the decomposition kinetics of bupivacaine in brain, blood and urine, which were collected from dogs executed by bupivacaine and stored in different conditions.
METHODS:Dogs were given arachnoid cavity anesthesia with bupivacaine. Then the brain, blood and urine were collected and divided equally to three groups stored in 20, 4 and -20 degrees C respectively. The concentrations of bupivacaine at different days were determined by the GC. The equation and half-time period of decomposition kinetics were imitated and calculated with WinNolin program.
RESULTS:The decomposition kinetics of bupivacaine in the dogs' brain, blood and urine were fit to the first order kinetics. The common equation was lgC = lgCo-kt/2.303 and k was the decomposition constant of first order reaction.
CONCLUSION:Bupivacaine in the brain, blood and urine specimens were found to be decomposed at various environments for storage. The higher temperature for storage, the faster of decomposition reaction.
