The effect of temperature on the postmortem redistribution of bupivacaine in epidural anesthesia death dogs.
- Author:
Gao-qin ZHANG
1
;
Da-li WANG
;
Ke-ming YUN
;
Da-ming ZHANG
Author Information
1. School of Forensic Medicine, Shanxi Medical University, Taiyuan 030001, China.
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Analgesia, Epidural;
Anesthetics, Local/pharmacokinetics*;
Animals;
Brain/metabolism*;
Bupivacaine/pharmacokinetics*;
Dogs;
Forensic Toxicology;
Gas Chromatography-Mass Spectrometry/methods*;
Liver/metabolism*;
Male;
Models, Animal;
Postmortem Changes;
Temperature;
Time Factors;
Tissue Distribution
- From:
Journal of Forensic Medicine
2010;26(2):112-115
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish the models of postmortem redistribution(PMR) in dogs with epidural anesthesia and to investigate the effect of temperature on the PMR of Bupivacaine.
METHODS:Eighteen male dogs were executed by epidural anesthesia with a dose of 5 mg/kg bupivacaine hydrochloride and randomly divided into three groups, room temperature (20-23 degrees C) group, 4 degrees C group and -20 degrees C group. The cardiac blood, peripheral blood, liver and cerebrum were collected at 0, 2, 4, 8, 24, 48, 72, 96, 120h postmortem. The contents of bupivacaine in those samples were analyzed by GC-NPD and GC-MS, the difference among three groups were compared.
RESULTS:The bupivacaine PMR of room temperature group was evident and complex in cardiac blood, peripheral blood and cerebrum. The PMR of 4 degrees C group was weaker and slower than that of normal temperature group. The bupivacaine PMR of the -20 degrees C group was the weakest in three groups.
CONCLUSION:PMR of bupivacaine will happen in epidural anesthesia death dogs, but it could be delayed or prevent by low temperature storage.
