The quantitative analysis of Ub and UbE1 in focus of traumatic epilepsy.
- Author:
Peng-xiang HE
1
;
Yun-lin TANG
;
Wei-lin CAI
;
Yong-hua HUANG
;
Chao FANG
;
Hua-lan JING
Author Information
1. Department of Forensic Medicine, Zhongshan Medical College, SUN Yat-sen University, Guangzhou 510080, China. seahe7@sina.com.cn
- Publication Type:Journal Article
- MeSH:
Blotting, Western;
Cerebral Cortex/pathology*;
Craniocerebral Trauma/pathology*;
DNA Primers;
Epilepsy, Post-Traumatic/pathology*;
Forensic Pathology;
Humans;
Neurons/pathology*;
RNA, Messenger/metabolism*;
Reverse Transcriptase Polymerase Chain Reaction;
Ubiquitin/metabolism*;
Ubiquitin-Activating Enzymes/metabolism*;
Up-Regulation
- From:
Journal of Forensic Medicine
2010;26(2):84-87
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the expression of ubiquitin proteasome system (UPS) in the traumatic epilepsy pathogenesis and its value in traumatic epilepsy by quantitative analysis.
METHODS:Fifteen specimens from human epileptic temporal cortex from PTE were collected as the PTE group. Fifteen specimens from non-PTE were collected as the non-PTE group. Fifteen normal cerebral cortex specimens died from acute traffic accident were collected as the control group. The expression of mRNA and protein of Ub and UbE1 were detected by RT-PCR and Western blot. Statistical analysis was used to compare the data between three groups.
RESULTS:The expression of mRNA and protein of Ub and UbE1 were the following order: PTE group(high), non-PTE group(middle) and control group(low).
CONCLUSION:The study confirms that UPS is up-regulated in the epilepsy's focus, especially in traumatic epilepsy. The activation of UPS may be an important pathological change in neurons in pathogenesis of traumatic epilepsy.
