Effects of cocaine on activities of ATPase, LDH and SDH in mouse splenocytes.
- Author:
Wen-ping SUN
1
;
Yan-xu LU
;
Xiao-yu ZHANG
;
Wei-wei TANG
;
Qing-yu HUANG
Author Information
1. Department of Forensic Pathology, Taishan Medical College, Taian 271000, China. wenpingsun@163.com
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Adenosine Triphosphatases/metabolism*;
Animals;
Cells, Cultured;
Cocaine/pharmacology*;
Dose-Response Relationship, Drug;
L-Lactate Dehydrogenase/metabolism*;
Male;
Mice;
Mice, Inbred Strains;
Spleen/enzymology*;
Succinate Dehydrogenase/metabolism*
- From:
Journal of Forensic Medicine
2010;26(2):81-83
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To examine the effects of cocaine on the activities of ATPase, LDH and SDH in cultured mouse splenocytes in vitro.
METHODS:The ATPase, LDH and SDH activities in mouse splenocytes were detected at day 7 after continuous culturing the mouse cells exposed to cocaine hydrochloride in final concentration of 10, 20 and 100 microg/mL in vitro.
RESULTS:The activities of ATPase, LDH and SDH in mouse splenocytes exposed to cocaine hydrochloride in final concentration of 10, 20 and 100 microg/mL were significantly decreased after continuous culturing for 7 days.
CONCLUSION:The present study demonstrated that cocaine could inhibit the activities of ATPase, LDH and SDH in cultured splenocytes in vitro.
