Estimation of postmortem interval using FTIR spectroscopy in rats' cardiac muscle.
- Author:
Ping HUANG
1
;
Chang-pei SU
;
Shi-song LI
;
Bo XING
;
Ya TUO
;
Qing-yang LU
;
Yong KE
;
Zhen-yuan WANG
Author Information
1. Shanghai Key Laboratory of Forensic Science, Institute of Forensic Science, Ministry of Justice, PRChina, Shanghai 200063, China.
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Animals;
Autopsy;
Fatty Acids/metabolism*;
Forensic Pathology;
Male;
Models, Animal;
Myocardium/metabolism*;
Nucleic Acids/metabolism*;
Postmortem Changes;
Rats;
Rats, Sprague-Dawley;
Spectroscopy, Fourier Transform Infrared/methods*;
Time Factors
- From:
Journal of Forensic Medicine
2010;26(1):1-5
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:FTIR (Fourier transform infrared) spectroscopy was applied to observe the process of postmortem degradation in rats' cardiac muscle and provided a new method for the estimation of post-mortem interval (PMI).
METHODS:The rats were sacrificed by cervical dislocation and the bodies were kept in a controlled environmental chamber set at (20 +/- 2) degrees C. The FTIR spectra was applied to measure the changes of different chemical group from rats' left ventricle muscle at the different time point postmortem.
RESULTS:There were not obvious changes for the main FTIR absorbance peaks. But the different FTIR absorbance at the wave-number (cm(-1)) indicated the three types: increase, decrease, stable. The various absorbance ratios also demonstrated the similar changes.
CONCLUSION:FTIR spectroscopy may be potentially used as an effective method for estimating PMI in forensic practice using cardiac muscle tissue.
