Effect of temperature and time on stability of ketamine in biological samples.
- Author:
Jie CAO
1
;
Zhen-Hua WANG
;
Juan JIA
;
Ke-Ming YUN
;
Zhi-Wen WEI
;
Yu-Jin WANG
Author Information
1. School of Forensic Medicine, Shanxi Medical University, Taiyuan 030001, China. cj9955@163.com
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Animals;
Brain/metabolism*;
Cryopreservation;
Disease Models, Animal;
Drug Stability;
Female;
Forensic Toxicology;
Gas Chromatography-Mass Spectrometry/methods*;
Hydrogen-Ion Concentration;
Ketamine/poisoning*;
Kidney/metabolism*;
Liver/metabolism*;
Male;
Rabbits;
Specimen Handling/methods*;
Temperature;
Time Factors
- From:
Journal of Forensic Medicine
2009;25(6):434-436
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:The stability of ketamine in biological samples was studied under different storage temperature and time.
METHODS:The rabbits were given intragastric administration of ketamine with a dose of 150 mg/kg and were sacrificed after 30 minutes. Blood, liver, kidney and brain of the rabbits were stored at room temperature (between 18 degrees C and 24 degrees C) and -20 degrees C. The specimens were analyzed at different times by GC-MS and GC-NPD.
RESULTS:At -20 degrees C, the concentration of ketamine decreased in all of samples (P < 0.05) within 30 days. The concentration of ketamine increased in all of samples stored at room temperature after 5 days(P < 0.05).
CONCLUSION:The stability of ketamine in biological samples stored at -20 degrees C was better than that at room temperature. The samples suspected containing ketamine should be stored at -20 degrees C and should be tested as soon as possible.
