Determination of ethyl glucuronide in blood by GC-MS/MS.
- Author:
Bao-Hua SHEN
1
;
Hui YAN
;
Hui ZHAO
;
Xian-Yi ZHUO
Author Information
1. Shanghai Key Laboratory of Forensic Medicine, Institute of Forensic Science, Ministry of Justice, PR China, Shanghai 200063, China.
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Alcohol Drinking/blood*;
Alcoholism/blood*;
Biomarkers/blood*;
Forensic Toxicology/methods*;
Gas Chromatography-Mass Spectrometry/methods*;
Glucuronates/blood*;
Humans;
Reproducibility of Results;
Sensitivity and Specificity
- From:
Journal of Forensic Medicine
2009;25(6):431-433
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To develop a method for determining ethyl glucuronide(EtG) in human blood with gas chromatograph-tandem mass spectrometry (GC-MS/MS).
METHODS:Human blood protein was precipitated with acetonitrile. The supernatant was transferred and air flow dried after centrifugated. The residue was derived with N, O-bis (trimethylsilyl)trifluoroacetamide (BSTFA), and analyzed with GC-MS/MS.
RESULTS:The detection limit of EtG in blood was 0.05 microg/mL. Calibration curve covered a span from 0.1-10 microg/mL with a good linear relationship (r = 0.999 9). The method showed a excellent performance when was used to authentic blood sample analysis for EtG.
CONCLUSION:The method is suitable for blood EtG analysis.
