Development and application of X-chromosomal STR multiplex amplification system.
- Author:
Qiu-Ling LIU
1
;
De-Jian LÜ
;
Hu ZHAO
Author Information
1. Department of Forensic Medicine, Zhongshan Medical College, Sun Yat-sen University, Guangzhou 510080, China. liuql@mail.sysu.edu.cn
- Publication Type:Journal Article
- MeSH:
Alleles;
Chromosomes, Human, X/genetics*;
DNA Primers;
Female;
Fluorescence;
Forensic Genetics/methods*;
Gene Amplification;
Gene Frequency;
Genetic Loci/genetics*;
Genotype;
Humans;
Microsatellite Repeats/genetics*;
Paternity;
Polymerase Chain Reaction/methods*;
Reproducibility of Results;
Sensitivity and Specificity
- From:
Journal of Forensic Medicine
2009;25(6):425-427
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the application of X-chromosomal STR(X-STR) for forensic identification and paternity testing.
METHODS:Six X-STR loci DXS6801, DXS9902, DXS6809, DXS6803, DXS6804 and DXS6799 were amplified in a single PCR reaction. PCR products were analyzed using capillary electrophoresis and 3100 Genetic Analyzer and GeneMapper ID v3.1 Analysis Software.
RESULTS:The alleles of all six X-STR loci were successfully obtained with unambiguous genotyping, high sensitivity and reproducibility.
CONCLUSION:The multiplex PCR of six X-STR loci is useful in forensic identification, particularly for sisters cases.
