Extract human DNA from maggot crop contents by phenol-chloroform method coupled with paramagnetic particle method.
- Author:
Xiang WANG
1
;
Ji-Feng CAI
;
Ming ZHONG
;
Jian-Bo LI
;
Jin-Guo ZHANG
;
Ling-Mei LAN
Author Information
1. Department of Forensic Medicine, College of Preclinical Medicine, Central South University, Changsha 410013, China. wangx6281@163.com
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Animals;
Cadaver;
Chloroform/chemistry*;
DNA/isolation & purification*;
DNA Fingerprinting/methods*;
Diptera/genetics*;
Entomology/methods*;
Forensic Sciences/methods*;
Gastrointestinal Contents;
Humans;
Larva/genetics*;
Phenol/chemistry*;
Polymerase Chain Reaction/methods*;
Sensitivity and Specificity;
Tandem Repeat Sequences
- From:
Journal of Forensic Medicine
2009;25(6):421-424
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish an effective phenol-chloroform method coupled with paramagnetic particle method for human DNA extraction from maggot crop contents in STR genotyping.
METHODS:Human DNA was extracted from the maggot crop contents using phenol-chloroform method and purified by paramagnetic particle method. DNA was quantified by PCR with Quantifiler Human DNA Quantification Kit using 7500 real-time fluorescence quantitative PCR instrument. PCR products were genotyped by AmpFlSTR Identifiler PCR Amplification Kit using 3130XL-Avant genetic analyzer.
RESULTS:The template DNA yield by the method described were increased at least 2 times than the phenol-chloroform extraction method alone. All of the full 16 STR profiles could be obtained with the samples extracted by this method when the DNA yield reached (0.218 +/- 0.041) ng/microL.
CONCLUSION:Phenol-chloroform method coupled with paramagnetic particle method can effectively increase the sensitivity of STR analysis of human DNA recovered from maggot crop contents and is a valuable tool for forensic entomology.
