Expression of FAP and alpha-SMA during the incised wound healing in mice skin.
- Author:
Yang GAO
1
;
Xue PENG
;
Zhan-Fen JIN
;
Zhi-Jun FU
Author Information
1. Department of Forensic Pathology, Harbin Medical University, Harbin 150081, China. gaoyangmail@126.com
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Actins/metabolism*;
Animals;
Blotting, Western;
Disease Models, Animal;
Endopeptidases;
Female;
Fibroblasts/metabolism*;
Forensic Pathology;
Gelatinases/metabolism*;
Immunohistochemistry;
Male;
Membrane Proteins/metabolism*;
Mice;
Random Allocation;
Serine Endopeptidases/metabolism*;
Skin/metabolism*;
Time Factors;
Wound Healing;
Wounds and Injuries/physiopathology*
- From:
Journal of Forensic Medicine
2009;25(6):405-408
- CountryChina
- Language:Chinese
-
Abstract:
UNLABELLED:OBJECTIVE To investigate the time-dependent expression of fibroblast activation protein (FAP) and alpha-smooth muscle actin(alpha-SMA) during the incised wound healing of the skin in mice.
METHODS:The expression of FAP and alpha-SMA in incised wound of mice skin was detected by immunohistochemistry and Western blot.
RESULTS:By immunohistochemistry, the expression of FAP and alpha-SMA in the normal skin and the skin 1 h after injury maintained at a very low level, but the positive cells expressing FAP and alpha-SMA started to elevate 6 h after injury and reached its peak on 5 d for FAP and on 3 d for alpha-SMA, then gradually decreased to the normal level on 14 d. The expression of FAP and alpha-SMA was observed throughout the wound healing stages 1 d after injuries by Western blot as well with a peak expression occurring on 5 d for FAP and on 3 d for alpha-SMA after injury.
CONCLUSION:FAP may be a potentially useful marker for wound age determination and alpha-SMA may be used as an effective indicator for the mid- and late stage incised wound of mice skin. The combination use of FAP and alpha-SMA may be potentially effective indicators for wound age determination.
