Expression of c-jun during the incised wound healing in mice skin.
- Author:
Me YANG
1
;
Da-Wei GUAN
;
Chang-Yan XIONG
;
Zi-Hui CHENG
;
Tian-Shui YU
Author Information
1. School of Forensic Medicine, China Medical University, Shenyang 110001, China. ymdoctor@163.com
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Animals;
Apoptosis;
Blotting, Western;
Disease Models, Animal;
Female;
Fibroblasts/metabolism*;
Immunohistochemistry;
Male;
Mice;
Neutrophils/metabolism*;
Proto-Oncogene Proteins c-jun/metabolism*;
Random Allocation;
Skin/metabolism*;
Time Factors;
Wound Healing;
Wounds and Injuries/metabolism*
- From:
Journal of Forensic Medicine
2009;25(6):401-404
- CountryChina
- Language:Chinese
-
Abstract:
UNLABELLED:OBJECTIVE To investigate the time-dependent expression of c-jun during the healing of incised wound in mice skin.
METHODS:The expression of c-jun in different stages after the incised wound were detected by immunohistochemistry and Western blot.
RESULTS:There was a low level expression of c-jun in normal mice skin. Expression of c-jun was mainly detected in neutrophils from 3 h to 12h after injury. The c-jun positive cells were almost mononuclear cells (MNCs) and fibroblasts between 1 d and 5 d after injury. The c-jun positive cells were mostly fibroblasts between 7 d and 14 d after injury. The ratio of the c-jun positive cells increased in the wound specimens from 3 h to 12 h, peaked at 12 h, declined partially from 1 d to 5 d, and reached the peak secondly at 7 d, then decreased from 10 d to 14 d. The expression of c-jun was observed throughout the wound healing stages by Western blot with two peaks occurring at 12 h and 7 d after injury.
CONCLUSION:The c-jun may play a potential role in inducing apoptosis of neutrophils, MNCs and fibroblasts during skin wound healing, and it may be used as the marker for wound age determination.
