Expression of Bcl-2 and Bax proteins in myocardial cells after Macleaya cordata alkaloids poisoning in rats.
- Author:
De-Yu ZHANG
1
;
Qing-Quan KONG
;
Chun-Bing ZHANG
;
Jian-Hua ZHU
;
Ming-Di CHEN
;
Peng WANG
Author Information
1. Institute of Forensic Science, Gongli Hospital of Pudong New District, Shanghai 200135, China. zhangdy32@126.com
- Publication Type:Journal Article
- MeSH:
Animals;
Disease Models, Animal;
Female;
Immunohistochemistry;
Male;
Myocardium/pathology*;
Myocytes, Cardiac/pathology*;
Papaveraceae/chemistry*;
Papaverine/poisoning*;
Proto-Oncogene Proteins c-bcl-2/metabolism*;
Rats;
Rats, Sprague-Dawley;
Staining and Labeling;
Time Factors;
bcl-2-Associated X Protein/metabolism*
- From:
Journal of Forensic Medicine
2009;25(4):246-253
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To observe the expression of Bcl-2 and Bax proteins in rat's myocardial cells after Macleaya cordata alkaloids poisoning, and to provide certain molecular biology references for the detection of Macleaya cordata alkaloids poisoning.
METHODS:Experimental model of Macleaya cordata alkaloids poisoning was established, the expression levels of Bcl-2 and Bax proteins in these cells were detected by immunohistochemistry, and the results were analyzed by computer image system.
RESULTS:The expression levels of Bcl-2 and Bax proteins in myocardial cells in poisoning groups were much greater than those in the control groups (P<0.05).
CONCLUSION:If the clinical symptoms may not be obvious, the detection of Bcl-2 and Bax proteins level by immunohistochemistry still could be ancillary method.
