Time-order expression of caspase-3 and iNOS in contused human brain tissue.
- Author:
Hong-Xing CAI
1
;
Zhou-Ru LI
;
Yan-Bo CHENG
;
Guo-Kai DONG
;
Jing-Fang ZHONG
;
Wen-Jiang YIN
Author Information
1. Department of Forensic Medicine, Xuzhou Medical College, Xuzhou 221002, China.
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Adult;
Astrocytes/metabolism*;
Brain/pathology*;
Brain Injuries/pathology*;
Caspase 3/metabolism*;
Female;
Forensic Pathology;
Humans;
Immunohistochemistry;
Male;
Middle Aged;
Neurons/metabolism*;
Nitric Oxide Synthase Type II/metabolism*;
RNA, Messenger/metabolism*;
Staining and Labeling;
Time Factors;
Young Adult
- From:
Journal of Forensic Medicine
2009;25(4):241-245
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the expression of caspase-3 and iNOS in different intervals and to provide evidence for estimation of injury intervals after brain contusion in human.
METHODS:Thirty cases died of serious brain injury were included into the injury groups and 5 cases died of non-brain injury were served as control group. To analyze the changes of caspase-3 and iNOS expression in brain samples at different intervals (2h, 4-8h, 10-14h, 1-2d, 3-5d, 8-11d) by immunohistochemistry and auto-image analysis system.
RESULTS:The level of caspase-3 expression started to increase in 2 hours after brain contusion compared to the control group (P<0.05). The level of caspase-3 expression continued to increase in 1-2 days and maintained high level in 3-5 days compared to the control group (P<0.05), then decreased gradually. There was no statistically significant difference between the expression level of iNOS in 2 hours with the control group (P>0.05). But the expression level of iNOS began to increase in 4-8 hours after brain contusion and reached its maximum in 1-2 days, then decreased. Weak expression of iNOS still could be detected in 8-11 days.
CONCLUSION:The expression of caspase-3 and iNOS can be used as effective evidence for human brain contusion interval.
