Changes of ICAM-1 mRNA expression in rats' skeletal muscle after contusion.
- Author:
Ming PEI
1
;
Jun-Hong SUN
;
Cai-Rong GAO
Author Information
1. School of Forensic Medicine, Shanxi Medical University, Taiyuan 030001, China. peiming5992@163.com
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Animals;
Contusions/pathology*;
Female;
Forensic Pathology;
Hindlimb/injuries*;
In Situ Hybridization, Fluorescence;
Intercellular Adhesion Molecule-1/metabolism*;
Male;
Muscle, Skeletal/metabolism*;
RNA, Messenger/metabolism*;
Random Allocation;
Rats;
Rats, Sprague-Dawley;
Time Factors;
Wound Healing
- From:
Journal of Forensic Medicine
2009;25(3):161-163
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the expression of intercellular adhesion molecular-1 (ICAM-1) mRNA in contused skeletal muscle of rats using fluorescence in situ hybridization (FISH) and its relationship with the contusion interval.
METHODS:To make the contusion models with rats skeletal muscle. The samples were taken to extract mRNA at 0.5 h, 1 h, 6 h, 12 h, 18 h, 24 h, 30 h and from the control group after contusion respectively. FISH was performed on frozen section samples and the sections were observed using LSCM.
RESULTS:The expression of ICAM-1 mRNA peaked at 6 h in skeletal muscle after contusion. Its level fell to 3.46 times the level of control group at 18 h and then increased again.
CONCLUSION:The time-order expression of ICAM-1 mRNA in 30 hours after contusion is potentially useful for estimation of early wound age.
