Simultaneous determination of clozapine, olanzapine and mirtazapine in human plasma by LC-MS/MS.
- Author:
Xian-Qin WANG
1
;
Xiao-Jun PAN
;
Guan-Yang LIN
;
Zheng XIANG
;
Xue-Bao WANG
;
Jian-Zhang WU
;
Dan LIN
Author Information
1. Analytical and Testing Center, Wenzhou Medical College, Wenzhou 325035, China. lankywang@163.com
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Benzodiazepines/blood*;
Chromatography, Liquid/methods*;
Clozapine/blood*;
Forensic Toxicology;
Humans;
Mianserin/blood*;
Mirtazapine;
Olanzapine;
Tandem Mass Spectrometry/methods*
- From:
Journal of Forensic Medicine
2009;25(2):123-126
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To develop a method for determination of clozapine, olanzapine and mirtazapine in human plasma by liquid chromatography-tandem mass spectrometry(LC-MS/MS).
METHODS:Clozapine, olanzapine and mirtazapine were extracted from plasma samples by using diethyl ether and separated by Agilent Zorbax SB-C18 column(2.1 mm x 150 mm, 5 microm). Electrospray ionization source was applied, positive ion mode was used to detect and multiple reaction monitoring mode was used to quantify clozapine, olanzapine and mirtazapine. Carbamazepine was the internal standard.
RESULTS:The detection limits of clozapine, olanzapine and mirtazapine were within 0.41-0.92 ng/mL. The calibration curve in the concentration range of 10.0-2000.0 ng/mL showed a good linear distribution (r > or = 0.992 4). The average extraction recoveries were within 65.7%-94.2%. Intra-day RSD and inter-day RSD were less than 6% (n = 5).
CONCLUSION:This method seems to be quite specific, sensitive and accurate, and can be used to detect clozapine, olanzapine and mirtazapine in forensic and clinical analytic toxicology.
