Comparing and evaluating six methods of extracting human genomic DNA from whole blood.
- Author:
Jing-Jing CHANG
1
;
Su-Hua ZHANG
;
Li LI
Author Information
1. Department of Forensic Medicine, Shanghai Medical College, Fudan University, Shanghai 200032, China.
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Chloroform/chemistry*;
DNA/isolation & purification*;
Forensic Medicine/methods*;
Genomics/methods*;
Humans;
Phenol/chemistry*;
Reagent Kits, Diagnostic;
Resins, Synthetic/chemistry*
- From:
Journal of Forensic Medicine
2009;25(2):109-114
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:Comparing the differences in purity and yield among six methods of extracting human genomic DNA from whole blood, which included Classic Phenol-chloroform extraction, modified combined technique composed of improved Phenol-chloroform extraction and Chelex-100 extraction, Chelex-100 extraction, IQ, Qiagen and SP.
METHODS:Ten samples of intravenous whole blood (5 mL/sample) were collected and human genomic DNA was extracted with these six methods. The purity and concentration of the DNA products were detected by ultraviolet spectrophotometry and fluorescent quantitation technique, the yield was calculated and tested with statistical software.
RESULTS:The Chelex-100 extraction was inferior in DNA purity to other methods while the other five methods showed no statistical difference. Modified combined technique was the poorest and IQ was the best in yield among the six methods of extraction. Statistical result showed that the extraction with high quality kits was better than that with classic Phenol-chloroform extraction, Chelex-100 extraction and modified combined technique composed of improved Phenol-chloroform. There was statistical difference between them.
CONCLUSION:Comparing to Phenol-chloroform extraction and Chelex-100 extraction, high quality kits are more useful in DNA extraction from forensic materials.
