Construction of standard allelic ladder of miniSTR loci by molecular cloning.
- Author:
Xue BAI
1
;
Bin CONG
;
Shu-Jin LI
;
Zhi-Ping HOU
;
Jian-Li GU
;
Ning LIU
;
Xia LI
;
Xia GUO
Author Information
1. Hebei Key Laboratory of Forensic Medicine, Department of Forensic Medicine, Hebei Medical University, Shijiazhuang 050017, China. snowhome2004@126.com
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Alleles;
Asian People/genetics*;
China/ethnology*;
Cloning, Molecular;
Forensic Genetics;
Gene Frequency;
Genetics, Population;
Humans;
Polymerase Chain Reaction;
Polymorphism, Genetic/genetics*;
Tandem Repeat Sequences/genetics*
- From:
Journal of Forensic Medicine
2009;25(2):106-108
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the genetic polymorphism of five miniSTR loci (D9S2157, D9S1122, D10S1435, D12ATA63, D2S1776) in Hebei Han population and to construct standard allelic ladders.
METHODS:Polymorphism of the five miniSTR loci in 120 unrelated individuals was analyzed by fluorescence PCR and ABI 310 Genetic Analyzer. Molecular cloning technique was employed to construct standard allelic ladder of the 5 loci.
RESULTS:Of the five miniSTR loci, 8, 8, 7, 5 and 8 alleles were found, respectively. The polymorphism information component were 0.790, 0.720, 0.750, 0.630 and 0.850, respectively.
CONCLUSION:The five loci have relatively abundant polymorphic information and their standard allelic ladders constructed by molecular cloning technique are useful in forensic science.
