The application of three methods combined for extracting DNA from human epithelium.
- Author:
Yan-xia GUO
1
;
Song CHEN
;
Kai-hui LIU
Author Information
1. Forensic Science Institute of Public Security Ministry, Beijing 100038, China.
- Publication Type:Journal Article
- MeSH:
DNA/isolation & purification*;
Epithelial Cells/cytology*;
Forensic Genetics;
Humans;
Polymerase Chain Reaction/methods*;
Resins, Synthetic/chemistry*
- From:
Journal of Forensic Medicine
2009;25(1):42-43
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To improve DNA extraction from human epithelium.
METHODS:Chelex-100, Chelex-100 organic combined and Chelex-100 magnetism pearl combined methods were adopted according to the situation of each case.
RESULTS:With the stepwise extracting protocol, the trace amount of DNA was analyzed most efficiently and the success rate of DNA extracting was better improved.
CONCLUSION:The efficiency of DNA extraction from human epithelium could be greatly improved by modifying the extracting protocol such as purification and concentration based on previous results.
